Entire Bandori cast (everyone is confirmed lesbian but this is surprisingly not well-known in the fandom)

Minori from Project SEKAI (sapphic)

Mizuki from Project SEKAI (transgirl)

Troy and Benson from Kipo and the Age Of Wonderbeasts (pansexual and gay)

Lily from Zombieland Saga (transgirl)

Barney from Dead End: Paranormal Park (gay transman)

Kazui from MILGRAM (gay)

Kaoru from Wonder Egg Priority (bisexual transman)

Homura from Madoka Magica (lesbian)